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...optical transfer function (OTF) of a microscope and is used to deconvolve images in fourier space. ...own i2i image format. We use a program called mmtoi2i to convert µManager images acquired on TESM to i2i files.

Converting from SL to Widefield images

...systematically identifying all individual endosomal structures within the images and #Images of TagRFP-T-clathrin are convolved using a difference of Gaussians (DOG) f

images; perform global thresholding, etc.) to the complex (e.g., deformable models ...random objects in a volume. Objects can either be individual voxels, tiny images, or connected voxels in an image.

...es. A custom TESM imaging protocol needs to be used in order to acquire SL images. The grating needs to be removed if you want to do pure Epi-fluorescence im ...tps://micro-manager.org/wiki/ITC18 ITC18] The ITC18 allows TESM to acquire images at high speed with precision, keeping all hardware devices synchronized. TE

All the images from these microscopes can still benefit from proper [[Software|processing]

[http://big.umassmed.edu/~lml/DaveImages/daveimages_http.html '''DAVE images'''] are viewable online. It is used to create test images for Fischer/hmc.

images; perform global thresholding, etc.) to the complex (e.g., deformable models The following narratives describe some of the BIG software, including images and references. The first 6 include a link to a separate web page which pro