Curriculum Vitae - Lawrence M. Lifshitz
Education
Professional History
2002-present: Associate Professor of Physiology and member of the Biomedical Imaging Group, University of Massachusetts Medical School, Worcester, Massachusetts.
1987-2002: Assistant Professor of Physiology and Nuclear Medicine and member of the Biomedical Imaging Group, University of Massachusetts Medical School, Worcester, Massachusetts.
1980 - 1987: Research Assistant in Graphics and Computer Vision at The University of North Carolina at Chapel Hill.
Publications
Zou H, Lifshitz LM, Tuft RA, Fogarty KE, Singer JJ, “Using total fluorescence increase (signal mass) to determine the Ca2+ current underlying localized Ca2+ events”, J. Gen. Physiol., Aug. 2004, 124:259-272.
Huang S, Lifshitz L, Patki-Kamath V, Tuft R, Fogarty K, Czech MP, “Phosphatidylinositol-4,5-bisphosphate-rich plasma membrane patches organize active zones of endocytosis and ruffling in cultured adipocytes”, Mol Cell Biol, 24(20):9102-23 (2004).
ZhuGe R, Fogarty KE, Baker SP, McCarron JG, Tuft RA, Lifshitz LM, Walsh Jr., JV, “Ca2+ spark sites in smooth muscle cells are numerous and differ in number of ryanodine recepors, BK channels and coupling ratio between them”, Am J Physiol, Cell Physiology, 287(6):C1577-88, Dec. 2004.
De Crescenzo V, ZhuGe R, Velazquez-Marrero C, Lifshitz LM, Custer E, Carmichael J, Lai FA, Tuft RA, Fogarty KE, Lemos JR, Walsh JV Jr. “Ca2+ syntillas, miniature Ca2+ release events in terminals of hypothalamic neurons, are increased in frequency by depolarization in the absence of Ca2+ influx”. J Neurosci. 2004 Feb 4;24(5):1226-35.
Lawe DC, Sitouah N, Hayes S, Chawla A, Virbasius JV, Tuft R, Fogarty K, Lifshitz L, Lambright D, Corvera S. “Essential role of Ca2+/calmodulin in Early Endosome Antigen-1 localization”. Mol Biol Cell. 2003 Jul;14(7):2935-45. Epub 2003 Mar 20.
Femino AM, Fogarty K, Lifshitz LM, Carrington W, Singer RH. “Visualization of single molecules of mRNA in situ”. Methods Enzymol. 2003;361:245-304.
Zou H, Lifshitz LM, Tuft RA, Fogarty KE, Singer JJ. “Visualization of Ca2+ entry through single stretch-activated cation channels”. Proc Natl Acad Sci U S A. 2002 Apr 30;99(9):6404-9.
Lawe, D., A. Chawla, E. Merithew, J. Dumas, W. Carrington, K. Fogarty, L. Lifshitz, R. Tuft, D.Lambright, and S. Corvera," Sequential roles for phosphatidylinositol 3-phosphate and Rab5 in tethering and fusion of early endosomes via their interaction with EEA1",J. Biol. Chem., 277(10), March 8, 2002, pp. 8611-8617.
Miyazaki K, Yano T, Schmidt DJ, Tokui T, Shibata M, Lifshitz LM, Kimura S, Tuft RA, Ikebe M., "Rho dependent agonist induced spatio-temporal change in myosin phosphorylation in smooth muscle cells", J. Biol. Chem, 277(1), Jan. 4, 2002, pp. 725-734.
Stachelek, S., R. Tuft, L. Lifschitz [sp] D. Leonard, A. Farwell, and J. Leonard, "Real-time Visualization of Processive, Myosin 5a-mediated Vesicle Movement in Living Astrocytes", J. Biol. Chem., 276(38), Sept. 21, 2001, pp.35652-35659.
Kirber, M.T., E.F. Etter, K.A. Bellvé, L.M. Lifshitz, R.A. Tuft, F.S. Fay, J.V. Walsh, Jr., and K.E. Fogarty. "The Relationship of Ca2+ Sparks to STOCs Studied with 2D and 3D Imaging in Feline Oesophageal Smooth Muscle Cells". Journal of Physiology, vol. 531(pt. 2), March, 2001, pp. 315-327.
Patki, V., J. Buxton, A. Chawla, L. Lifshitz, K. Fogarty, W. Carrington, R.Tuft, and S. Corvera. "Insulin action on GLUT4 traffic visualized in single 3T3-L1 adipocytes using ultra-fast microscopy", Molecular Biology of the Cell, vol. 12(1), January, 2001, pp. 129-141.
ZhuGe, R., K.E. Fogarty, R.A. Tuft, L.M. Lifshitz, K.Sayer and J.V. Walsh, Jr. "Dynamics of Signaling between Ca2+ Sparks and Ca2+ activated K+ Channels Studied with a Novel Image-based Method for Direct Intracellular Measurement of Ryanodie Receptor Ca2+ Current", Journal of General Physiology, vol. 116, 2000, pp 845-864.
Zou, H., L. Lifshitz, R. Tuft, K. Fogarty, and J. Singer, “Imaging Ca2+ Entering the Cytoplasm through a Single Opening of a Plasma Membrane Cation Channel”, Journal of General Physiology, vol. 114, Oct. 1999, pp. 1-14. Cover photo produced with DAVE.
Meininger, G., E. Moore, D. Schmidt, L. Lifshitz, and F. Fay, “Distribution of Active Protein Kinase C in Smooth Muscle”, Biophysical Journal, vol. 77, May, 1999, pp. 973-984.
Kiefer, C., J. McKenney, J. Trainor, R. Lambrecht, H. Bonkovsky, L. Lifshitz, C. Valeri, and L. Snyder, “Porphyrin Loading of Lipofuscin Granules in Inflammed Striated Muscle”, American Journal of Pathology, Vol. 153(3), September, 1998.
Rizzuto, R., P. Pinton, W. Carrington, F. Fay, K. Fogarty, L. Lifshitz, R. Tuft, and T. Pozzan,”Close Contacts with the Endoplasmic Reticulum as Determinants of Mitochondrial Ca2+ Responses”, Science, Vol. 280, June 12, 1998, pp. 1763-1766.
Lifshitz, L. M., “Determining Data Independence on a Digitized Membrane in Three Dimensions”, IEEE Transactions on Medical Imaging, Vol. 17(2), April 1998, pp. 299-303.
Bassell G., H. Zhang, A. Byrd, A. Femino, R. Singer, K. Taneja, L. Lifshitz, I. Herman, and K. Kosik,”Sorting of beta-Actin mRNA and Protein to Neurites and Growth Cones in Culture”, Journal of Neuroscience, Vol. 18(1), January, 1998, pp.251-265.
Fay, F. S., K. L. Taneja, S. Shenoy, L. M. Lifshitz, and R. H. Singer, “Quantitative digital analysis of diffuse and concentrated distributions of nascent transcripts, SC35 and Poly(A)”, Experimental Cell Research (with cover photo), Vol. 231(1), February 25, 1997, pp. 27-37.
Lifshitz, L. M., J. Collins, E. Moore, J. Gauch, "Computer Vision and Graphics in Fluorescence Microscopy". In: Proceedings of the IEEE Workshop on Biomedical Image Analysis, IEEE Computer Society Press, Los Alamitos, CA, 1994, pp. 166-175.
Moore, E., E. Etter, K. Philipson, W. Carrington, K. Fogarty, L. M. Lifshitz, and F. S. Fay, “Coupling of the Na+/Ca+2 Exchanger to the Na+/K+ Pump and Sarcoplasmic Reticulum in Smooth Muscle”, Nature, Vol. 365, October 14, 1993, pp. 657-660.
Taneja, K. L., L. M. Lifshitz, F. S. Fay, and R. H. Singer, “Poly(A) RNA Codistribution with Microfilaments: Evaluation by In Situ Hybridization and Quantitative Digital Imaging Microscopy”, The Journal of Cell Biology, Vol. 119(5), December, 1992, pp. 1245-1260.
Lifshitz, L. M.,K. Fogarty, J. M. Gauch, and E. Moore, “Computer Vision and Graphics in Fluorescence Microscopy”, SPIE Proceedings, vol. 1808, Visualization in Biomedical Computing 1992, R. A. Robb (ed.), October, 1992, pp. 521-534.
Lifshitz, L. M., “Model-Based Tracking of Deformable Filaments”, SPIE Proceedings, vol. 1609, Model-Based Vision Development and Tools, R. M. Larson and H. N. Nasr (eds.), November, 1991, pp. 185-197.
Lifshitz, L. M. and S. M. Pizer, “A Multiresolution Hierarchical Approach to Image Segmentation Based on Intensity Extrema”, IEEE Transactions on Pattern Analysis and Machine Intelligence. Vol. 12, No. 6, June, 1990, pp. 529-540.
Lifshitz, L. M., F. S. Fay, S. Gilbert, K. Fogarty, and W. Carrington, “Tracking cellular features using motion constraints and global information”, SPIE Proceedings, vol. 1205, Bioimaging and Two Dimensional Spectroscopy, L. C. Smith (ed.), January, 1990, pp. 93-103.
Fay, F. S., W. Carrington, L. M. Lifshitz and K. Fogarty, “Three- dimensional analysis of molecular distribution in single cells using the digital imaging microscope”, SPIE Proceedings, vol. 1161, New Methods in Microscopy and Low Light Imaging, J. E. Wampler (ed.), August, 1989, pp. 12-23.
Carrington, W., K. E. Fogarty, F. S. Fay, and L. M. Lifshitz, “Analysis of 3-D Molecular Distribution with the Digital Imaging Microscope”, Proceedings of the 46th Annual Meeting of the Electron Microscopy Soc. of America, San Francisco Press, 1988, pp. 40-41.
Pizer, S. M., J. Gauch, and L. M. Lifshitz, “Interactive 2-D and 3-D Object Definitions in Medical Images Based on Multiresolution Image Descriptions”, SPIE Conference on Medical Imaging, February, 1988.
Lifshitz, L. M., and S. M. Pizer, “A Multiresolution Hierarchical Approach to Image Segmentation based on Intensity Extrema”. In: Information Processing in Medical Imaging, Proceedings of the 10th International Conference, Plenum Press, N.Y., 1988, pp. 107-130.
Pizer, S. M., H. Fuchs, C. Mosher, L. Lifshitz, G. Abram, Ramanathan, Whitney, Rosenman, Staab, Chaney and Sherouse, “3D Shaded Graphics in Radiotherapy and Diagnostic Imaging”, Proceedings of Computer Graphics '86, National Computer Graphics Association, 1986.
Pizer, S. M., J. J. Koenderink, L. M. Lifshitz, L. Helmink and A. D. J. Kaasjager, “An Image Description for Object Definition, Based on Extremal Regions in the Stack”. In: Information Processing in Medical Imaging, Proceedings of the ninth conference, Kluwer Academic Publishers, 1986, pp. 24-37.
Jaques, P., F. DiBianca, S. M. Pizer, F. Kohout, L. M. Lifshitz, and D.Delany, “Quantitative Digital Fluorography - Computer vs Human Estimation of Vascular Stenoses”, Investigative Radiology, January, 1985, pp. 42-52.
Book Chapters or Sections
Carrington, W., K. Fogarty, R. Rizzuto, L. Lifshitz, and R. Tuft, “High Resolution 3-D Imaging of Living Cells by Image Restoration”, in Imaging Living Cells, R. Rizzuto and C. Fasolato, eds., Springer, Heidelberg, 1998.
Lifshitz, L. M., “Tracking Cells and Subcellular Features”. In: Advances in Image Analysis, Y. Mahdavieh and R.C. Gonzalez (eds.), SPIE Press, 1992, pp. 218-243.
Lifshitz, L. M. and S. M. Pizer. “A Multiresolution Hierarchical Approach to Image Segmentation Based on Intensity Extrema”. In: Computer Vision: Advances and Applications, R. Kasturi and R. Jain (eds.), IEEE Computer Society Press, 1991, pp. 606-617.
Carrington, W., K. E. Fogarty, L. M. Lifshitz, and F. S. Fay, “Three Dimensional Imaging on Confocal and Wide Field Microscopes”. In: The Handbook of Biological Confocal Microscopy, J. Pawley, (ed.) Plenum Press, N.Y.,1990, pp. 151-161.
Fay, F. S., K. Fogarty, W. Carrington, and L. M. Lifshitz, “Extraction of Information from Biological Systems Using the Digital Imaging Microscope: Analysis of White Blood Cell Chemotaxis”. In: Optical Microscopy for Biology, B. Herman and K. Jacobson (eds.), Wiley-Liss Publishers, N.Y., 1990, pp. 419-435.
Abstracts
Carmichael, JD, DeCrescenzo, V, Fogarty, K, Lifshitz, LM, Lemos, JR, Lai, A, Walsh, JV., “Different Spatial Distribution of Type 1 and Type 2 Ryanodine Receptors in Nerve Terminals from Mouse Neuoreohypophysis”, Poster/Abstract Biophysical Society, 48th Annual Meeting, Baltimore, Feb., 2004.
DeCrescenzo, V., R. ZhuGe, C. Velaszquez-Marrero, L. Lifshitz, E. Custer, J. Carmichael, A. Lai, R. Tuft, K. Fogarty, J. Lemos, J. Walsh. “Miniature Ca2+ Release Events in Nerve Terminals (Ca2+ Syntillas) are Increased in Frequency by Depolarization in the Absence of Extracellular Ca2+”, poster at the Second International Symposium on Computational Cell Biology, March 23-25, 2003, Lenox, MA and also at the Biophysical Society, 47th Annual Meeting, San Antonio, March 1-5, 2003.
Femino, A., K. Fogarty, L. Lifshitz, E. Powrie, R. Tuft, R. Singer,. “High Resolution RNA Dynamics in Living Yeast”, poster at the Second International Symposium on Computational Cell Biology, March 23-25, 2003, Lenox, MA.
Zou, H., L. Lifshitz, M. Kirber, R. Tuft, K. Fogarty, J. Singer, "Visualization of Ca2+ Entry Through Stretch-Activated Cation Channels", Biophysical Journal 80:1 A503, Biophysical Society, 45th Annual Meeting, Boston, Feb. 17-21, 2001.
ZhuGe, R., K. Fogarty, S. Baker, R. Tuft, L. Lifshitz, J. Walsh, “Individual Ca2+ Spark Sites in Smooth Muscle Differ in Number of Ryanodine Receptors and BK Channels ”, Biophysical Journal 80:1 A301, Biophysical Society, 45th Annual Meeting, Boston, Feb. 17-21, 2001.
Patki, V., J. Buxton, A. Chawla, L. Lifshitz, K. Fogarty, W. Carrington, R.Tuft, and S. Corvera. "Insulin action on GLUT4 traffic visualized in single 3T3-L1 adipocytes using ultra-fast microscopy", poster at the American Society for Cell Biology Annual Meeting, Dec 9-13, 2000. San Francisco, CA. The abstract appears in a supplement to Mol. Biol. Cell 11S:127a, Dec 2000.
Carmichael, J., R. Ikebe, J. Saito, N. Saiki, L. Lifshitz, M. Ikebe, “Differences in Actin binding between Calponin and its phosphorylation-site mutants”, Biophysical Society, 44th Annual Meeting, New Orleans, Feb. 12-16, 2000.
Carrington, W., R. Tuft, L. Lifshitz, K. Fogarty, “3-D Fluorescent Imaging: 100 Nanometer Resolution at 500 Frames/Second”, Biophysical Society, 44th Annual Meeting, New Orleans, Feb. 12-16, 2000 (and platform presentation by W. Carrington).
ZhuGe, R., R. Tuft, L. Lifshitz, K. Fogarty, J. Walsh, “Functional Coupling Ratio Between Ryanodine Receptors and BK Channels is Variable, Causing Ca2+ Sparks of the Same Intensity to Elicit STOCS of Different Amplitude”, Biophysical Society, 44th Annual Meeting, New Orleans, Feb. 12-16, 2000 (and platform presentation by K. Fogarty).
Zou, H., L.M. Lifshitz, R.A. Tuft, K.E. Fogarty, and J.J. Singer. “Imaging Ca2+ entry due to a single opening of a plasma membrane cation channel”. Biophysical Journal 76: A465, 1999.
Lifshitz, L., E. Etter, K. Fogarty, K. Bellve, R. Tuft, J. Walsh, and M. Kirber, “Monte Carlo Simulation Indicates Non-Random Spatial Distribution of Ca2+ Sparks in Smooth Muscle”, 1998 Proceedings of the Society of General Physiologists (Sept. Woods Hole meeting), to be published in the Journal of General Physiology. (Also a poster)
Keifer, C., J. McKenney, J. Trainer, L. Lifshitz, S. Krasnicki, T. Schneider, S. Schrier, J. Morrow, C. Valeri, and L. Snyder, ”Hemoglobin-Spectrin Complexes: Distribution within the Erythrocyte Skeleton”, 37th meeting of The American Society of Hematology, Seattle, Washington, 1995.
Moore, E., W. Carrington, K. Fogarty, L. Lifshitz, and F. Fay, "Interpolated Image Deconvolution and Colocalization Analysis", Biophysical Society Conference, 1995.
Ferris, C., Y. Delville, J. Collins, and L. Lifshitz, "Imaging Synaptic Connectivity with Widefield Digital Microscopy", Society for Neuroscience Abstracts, vol. 20, part 2 (24th Annual Neuroscience Conference), p. 1176 (also a poster), 1994.
Gilbert, S., L. Lifshitz, and F. S. Fay, “Feature analysis of phase contrast images of newt eosinophil chemotaxis”,Physiologist, 32:211a. 1989.
Meininger, G. A., L. M. Lifshitz, and F. S. Fay, “Distribution of Protein Kinase C in Smooth Muscle”, ASCB (Cell Biology) Conference, December, 1991.
Posters/Presentations
DeCrescenzo, V., R. ZhuGe, C. Velaszquez-Marrero, L. Lifshitz, E. Custer, J. Carmichael, A. Lai, R. Tuft, K. Fogarty, J. Lemos, J. Walsh. “Miniature Ca2+ Release Events in Nerve Terminals (Ca2+ Syntillas) are Increased in Frequency by Depolarization in the Absence of Extracellular Ca2+”, poster at the Second International Symposium on Computational Cell Biology, March 23-25, 2003, Lenox, MA and also at the Biophysical Society, 47th Annual Meeting, San Antonio, March 1-5, 2003.
Femino, A., K. Fogarty, L. Lifshitz, E. Powrie, R. Tuft, R. Singer,. “High Resolution RNA Dynamics in Living Yeast”, poster at the Second International Symposium on Computational Cell Biology, March 23-25, 2003, Lenox, MA.
ZhuGe, R., K. Fogarty, L. Lifshitz, R. Tuft, J. Walsh, “Kinetic Relationship Between Ca2+ Currents Underlying Ca2+ Sparks and Corresponding STOCS in Airway Myocytes”, platform presentation by R. ZhuGe at the Biophysical Society, 47th Annual Meeting, San Antonio, March 1-5, 2003.
Carrington, W., K. Bellve, R. Tuft, L. Lifshitz, K. Fogarty, “High Speed 4-d Fluorescence Microscopy of Live Cells”, poster at the Biophysical Society, 47th Annual Meeting, San Antonio, March 1-5, 2003.
Bellve, K., K. Fogarty, L. Lifshitz, W. Carrington, R. Tuft,, “Design of a Fluorescence Digital Microscope System for Time-Resolved, Fast 3-D Imaging of Intracellular Molecular Dynamics”, poster at the Biophysical Society, 47th Annual Meeting, San Antonio, March 1-5, 2003.
Lifshitz, L., E. Rapizzi, J. Carmichael, R. Tuft, K. Fogarty, “Quantitative Analysis of Colocalization of Multiple Molecular Distributions Using Digital Fluorescence Imaging Microscopy”, poster at the Biophysical Society, 47th Annual Meeting, San Antonio, March 1-5, 2003.
Lifshitz, L. M., K. Taneja, R. Singer, and F. S. Fay, "Quantitative Evaluation of the Extent of Colocalization between Two Images", Cell Biology Conference, 1992. Voted best poster in topic area.
Lifshitz, L. M., "Tracking Deformable Filaments", Image Processing in Medical Imaging '91, Kent, England, July 7-12, 1991.
Other
Dictenberg,J., W. Zimmerman, C. Sparks, A. Young, C. Vidair, Y. Zheng, W. Carrington, F. Fay, and S. Doxsey, “Pericentrin and Gamma-Tubulin Form a Protein Complex and are Organized into a Novel Lattice at the Centrosome”, Journal of Cell Biology, vol. 141(1), April 6, 1998, pp. 163-174. Cover photo rendered using Data Analysis and Visualization Environment (DAVE) developed by L. Lifshitz.
Femino, A.M., F.S. Fay, K. Fogarty, and R.H. Singer, “Visualization of Single RNA Transcripts in Situ”, Science, April 24, 1998, vol. 280, pp. 585-590. Cover photo (and others) rendered using Data Analysis and Visualization Environment (DAVE) developed by L. Lifshitz.
Cover photo of Experimental Hematology, vol. 26(7), July, 1998. Dual labelled stem cell rendered using DAVE.
Shi, L. M. Lifshitz, and B. Brill, “The Use of Cluster Analysis Difference Imaging in Cancer Diagnosis Using Radiolabeled Monoclonal Antibodies”, Presented at the International Symposium on Nuclear Medicine, in Bejing, China, Oct. 10-14, 1988.
Pizer, S. M., J. M. Gauch, L. M. Lifshitz, and W. R. Oliver, “Image Description via Annihilation of Essential Structures”. Invited paper, Workshop in Computational Vision/Multiresolution Representation of Images, University of Copenhagen, November, 1987.
Affiliations
Member: IEEE, ACM
Ad Hoc: Past Member of NIH Shared Instrumentation Review Panel, Reviewer for Biophysical Journal.